This ICTP-IAEA workshop, tailored for medical physicists, delves into the complex world of nuclear imaging. Participants will explore SPECT & PET quantification, both theoretical and hands-on, using state-of-the-art freeware. Emphasis on quality protocols and addressing physical effects ensures data accuracy. The curriculum includes Monte Carlo simulations for gamma camera optimization and insights into the evolution of hybrid imaging. The goal of this course is to arm attendees with the skills and knowledge to leverage future innovations, ensuring a seamless blend of theory and practical application for improved clinical outcomes.